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Characterization and encapsulation of exosomes. (A) Morphology of exosomes tested by TEM. (B) Particle size analysis of exosomes by NTA. (C) Western blot results of tetraspanins CD9 and <t>CD63</t> expression in exosomes. (D) Three-dimensional structure of fibrin glue observed in SEM. (E) Three-dimensional distribution of CM-DiI-labeled exosomes (red) in the fibrin glue scanned by CLSM. The images showed views of different angles, and the spatial distribution of exosomes was presented by images in lower lines with pseudo colors according to position Z.
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Characterization and encapsulation of exosomes. (A) Morphology of exosomes tested by TEM. (B) Particle size analysis of exosomes by NTA. (C) Western blot results of tetraspanins CD9 and <t>CD63</t> expression in exosomes. (D) Three-dimensional structure of fibrin glue observed in SEM. (E) Three-dimensional distribution of CM-DiI-labeled exosomes (red) in the fibrin glue scanned by CLSM. The images showed views of different angles, and the spatial distribution of exosomes was presented by images in lower lines with pseudo colors according to position Z.
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Characterization and encapsulation of exosomes. (A) Morphology of exosomes tested by TEM. (B) Particle size analysis of exosomes by NTA. (C) Western blot results of tetraspanins CD9 and CD63 expression in exosomes. (D) Three-dimensional structure of fibrin glue observed in SEM. (E) Three-dimensional distribution of CM-DiI-labeled exosomes (red) in the fibrin glue scanned by CLSM. The images showed views of different angles, and the spatial distribution of exosomes was presented by images in lower lines with pseudo colors according to position Z.

Journal: Asian Journal of Pharmaceutical Sciences

Article Title: Rapid and effective treatment of traumatic spinal cord injury using stem cell derived exosomes

doi: 10.1016/j.ajps.2021.10.002

Figure Lengend Snippet: Characterization and encapsulation of exosomes. (A) Morphology of exosomes tested by TEM. (B) Particle size analysis of exosomes by NTA. (C) Western blot results of tetraspanins CD9 and CD63 expression in exosomes. (D) Three-dimensional structure of fibrin glue observed in SEM. (E) Three-dimensional distribution of CM-DiI-labeled exosomes (red) in the fibrin glue scanned by CLSM. The images showed views of different angles, and the spatial distribution of exosomes was presented by images in lower lines with pseudo colors according to position Z.

Article Snippet: The sample was centrifuged and examined in Jess Simple Western System (Bio-techne, Minneapolis, USA) using CD9 (OM198821, Omnimabs, CA, USA) and CD63 antibodies (OM245783, Omnimabs, CA, USA).

Techniques: Particle Size Analysis, Western Blot, Expressing, Labeling